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RNase H Molecular Tool Enzyme

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Guangzhou Dongsheng Biotech Co., Ltd

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RNase H Molecular Tool Enzyme

Name: RNase H Molecular Tool Enzyme
Brand: GDSBio
Price: 22 USD

Brand Name : GDSBio

Model Number : E1016

Certification : /

Place of Origin : China

MOQ : 100 U

Price : USD 22-29/100 U

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 100 Bag/Bags Per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Product Name : RNase H

Cat. No./Spec. : E1016-A/100 U, E1016-B/500 U

Appearance : colourless

Application : Removal of mRNA

Classification : General Reagents

Grade : molecular biology

Logo Printing : With Logo Printing

Transport Package : Packing

Production Capacity : 100 Bag/Bags Per Day

Storage Conditions : Store at -20°C

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RNase H

Cat. No./Spec.: E1016-A/100 U, E1016-B/500 U
Concentration: 5 U/µL

Product Description
Ribonuclease H (RNase H) can specifically degrade the RNA strand within RNA-DNA hybrids. This enzyme does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA and RNA.

Storage Condition & Shelf Life
Store at -20°C.

Source
E. coli strain MRE-600.

Unit Definition
A unit is defined as the amount of enzyme required to catalyze the formation of 1 nmol of acid-soluble products within 20 minutes at 37°C.
Enzyme activity is determined in the following mixture: 20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl2,1 mM DTT, 24 µM [3H]-poly(A)·poly(dT), 0.03 mg/mL BSA, and 4% (v/v) glycerol.

Scope of Application
- Removal of mRNA before the synthesis of the second-strand cDNA
- RT-PCR and qRT-PCR: Removal of RNA after the first-strand cDNA synthesis
- Removal of poly(A) sequence from mRNA after hybridization with Oligo(dT)
- Site-specific cleavage of RNA
- Study of products from in vitro polyadenylation reactions

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RNase H

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