Sign In | Join Free | My fnxradio.com
China Guangzhou Dongsheng Biotech Co., Ltd logo
Guangzhou Dongsheng Biotech Co., Ltd
Professional Supplier of PCR & NGS Reagents
Active Member

4 Years

Home > NGS Library Construction >

NGS Library Construction Fast DNA Library Prep Kit

Guangzhou Dongsheng Biotech Co., Ltd
Contact Now

NGS Library Construction Fast DNA Library Prep Kit

Brand Name : GDSBio

Model Number : K001-A, K001-B

Certification : ISO9001,ISO13485

Place of Origin : China

MOQ : 6 rxns

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 100000 rxns per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Cat. No. : K001-A, K001-B

Specification : K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns

Appearance : Complete, no damage

Stock : yes

Logo Printing : With Logo Printing

Transport Package : Packing

Adapter Ligation : Yes

Delivery Format : Plate/Tube

End Repair : Yes

Fragment Size Range : 150 bp – 500 bp

Fragmentation Method : Mechanical/Enzymatic

Library Pooling : Yes

Library Type : DNA

PCR Amplification : Yes

Product Name : NGS Library Construction

Sequencing Platform : Illumina

Contact Now

Fast DNA Library Prep Kit

Instruction for Use

【Product Name】

Fast DNA Library Prep Kit

【Cat. No./Spec.】

K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Application Sample Type Recommended Amount
Whole genome sequencing High quality complex genomes 50ng-1μg
Target capture sequencing of whole exome High quality complex genomes 10ng-1μg
Target capture sequencing of whole genome FFPE DNA ≥50ng
Target capture sequencing of whole genome cfDNA/ctDNA ≥100pg
Whole genome sequencing Microbial genome 1ng-1μg
Whole genome sequencing(PCR-free) High quality DNA

≥50ng (no size selection)

≥200ng (size selection)

ChIP-Seq ChIP DNA ≥100pg
Targeted sequencing Amplicon ≥100pg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component 24 rxns 96 rxns
End Repair & A-Tailing Mix 480 μl 4×480 μl
Fast DNA Ligase 120 μl 2×240 μl
Fast Ligation Buffer 600 μl 4×600 μl
2× HIFI Library PCR Master Mix 600 μl 4×600 μl
Primer Mix* 120 μl 480 μl

* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index.

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.

NGS Library Construction Fast DNA Library Prep Kit

NGS Library Construction Fast DNA Library Prep Kit


Product Tags:

Saliva Rapid Viral Antigen Test

      

Flu B Rapid Viral Antigen Test

      

Flu A antigen saliva test kit

      
Wholesale NGS Library Construction Fast DNA Library Prep Kit from china suppliers

NGS Library Construction Fast DNA Library Prep Kit Images

Inquiry Cart 0
Send your message to this supplier
 
*From:
*To: Guangzhou Dongsheng Biotech Co., Ltd
*Subject:
*Message:
Characters Remaining: (0/3000)