RNasin (RNase Inhibitor) #R2011 1,000U
RNasin (RNase Inhibitor)
Components
| Component | R2011 (1,000 U) |
| Concentration | 40U/μl |
Store at -20°C
For research only
In total 1 vial.
Description
RNasin is a ribonuclease inhibitor purified from human placenta. RNasin reduces the activity of RNase A-type enzymes in a variety of organisms.
Source
E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.
Storage Buffer
20mM HEPES-NaOH (pH7.5), 50mM NaCl, 8mM DTT, 0.5mM ELUGENT Detergent and 50% (v/v) glycerol.
Feature
Free of endodeoxyribonucleases, exodeoxyribonucleases, rib- onucleases and phosphatases.
Definition of Activity Unit
One unit of the protein inhibits the activity of 5ng RNase A by 50%.
Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml E.coli [3H]-RNA, 50 mg/ml yeast RNA, 8 mM DTT.
Applications
• Inhibition of RNA degradation in the following procedures:
-in vitro transcription;
-cDNA synthesis;
-in vitro translation;
-isolation of mammalian cell fractions that contain mRNA
protein complex.
• Separation and identification of specific ribonuclease activities.
• Studies of tumor suppression.
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