Hotstart Pfu Mix
Hotstart Pfu Mix PCR Master Mix P2051 P2052
Cat. No.: P2051, P2052
Contents:
| Component | P2051 | P2052 |
| 2X Hotstart Pfu Mix | 1 ml | 1 ml × 5 |
| Nuclease-free water | 1 ml | 1 ml × 5 |
Store at -20°C
In total 10 vials.
Description
2X Hotstart Pfu Mix is a premixed, ready-to-use solution containing Hotstart Pfu DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. The mix retains all features of Hotstart Taq DNA Polymerase.
Hotstart Pfu DNA Polymerase is a hot-start polymerase with antibody-like modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length can reach to 5 kb (simple template) with the elongation rate of 2kb/min. Hotstart Taq has 5’-3’ polymerase activity and 3’-5’ exonuclease activity. There are blunt-end and 3’dA-end two kinds of products. Hotstart Pfu DNA Polymerase has zero animal source pollution by being produced with advanced antibody-like modification. And it is much more stable than antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerases and the initial-denaturation time can be reduced to 3 minutes.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Composition of the 2X Hotstart Pfu Mix
0.5U/ul Hotstart Pfu DNA Polymerase, 2× PCR buffer, 0.4mM dNTPs, 3.2mM MgCl2, 0.02% bromophenol blue (optional).
Applications
• High-specificity amplification & multiplex PCR: hot-start polymerase with antibody-like modification
• High fidelity PCR
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
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Hotstart Pfu Mix PCR Master Mix P2051 1m P2052 Blue Buffer Images
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