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GDSBio NGS Library Construction Fast DNA Library Prep Kit V2

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Guangzhou Dongsheng Biotech Co., Ltd

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GDSBio NGS Library Construction Fast DNA Library Prep Kit V2

Name: GDSBio NGS Library Construction Fast DNA Library Prep Kit V2
Brand: GDSBio
Price: 1 USD

Wholesale GDSBio NGS Library Construction Fast DNA Library Prep Kit V2 from china suppliers

Brand Name : GDSBio

Model Number : K001S-A, K001S-B

Certification : ISO9001, ISO13485

Place of Origin : China

MOQ : 1 box

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 10000 Box/Boxes per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Cat. No. : K001S-A, K001S-B

Specification : K001S-A/24 rxns; K001S-B/96 rxns; sample sack/ 6 rxns

Appearance : clear liquid

Stock : yes

Library Type : Amplicon/Targeted/Whole Genome/Exome

Sample Type : DNA

Logo Printing : With Logo Printing

Transport Package : Packing

Sequencing Platform : Illumina

Storage Conditions : Stored at -20°C, with a validity period of 12 months.

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Fast DNA Library Prep Kit V2

【Product Name】

Fast DNA Library Prep Kit V2

【Cat. No./Spec.】

K001S-A/24 rxns; K001S-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Application	Sample Type	Recommended Amount
Whole genome sequencing	High quality complex genomes	50ng-1μg
Target capture sequencing of whole exome	High quality complex genomes	10ng-1μg
Target capture sequencing of whole genome	FFPE DNA	≥50ng
Target capture sequencing of whole genome	cfDNA/ctDNA	≥100pg
Whole genome sequencing	Microbial genome	1ng-1μg
Whole genome sequencing(PCR-free)	High quality DNA	≥50ng (no size selection) ≥200ng (size selection)
ChIP-Seq	ChIP DNA	≥100pg
Targeted sequencing	Amplicon	≥100pg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component	24 rxns	96 rxns
End Repair & A-Tailing Enzyme Mix	120 μl	2×240 μl
End Repair & A-Tailing Buffer	240 μl	2×480 μl
Fast DNA Ligase	120 μl	2×240 μl
Fast Ligation Buffer	600 μl	4×600 μl
2× HIFI Library PCR Master Mix	600 μl	4×600 μl
Primer Mix*	120 μl	480 μl

* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index.

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.

GDSBio NGS Library Construction Fast DNA Library Prep Kit V2

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

GDSBio NGS Library Construction Fast DNA Library Prep Kit V2

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