NGS Library Construction Fast DNA Library Prep Kit K001-A K001-B
【Product Name】
Fast DNA Library Prep Kit
【Cat. No./Spec.】
K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns
【Product Description】
Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and end “A” tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end repair, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.
【Sample Type】
| Application | Sample Type | Recommended Amount |
| Whole genome sequencing | High quality complex genomes | 50ng-1μg |
| Target capture sequencing of whole exome | High quality complex genomes | 10ng-1μg |
| Target capture sequencing of whole genome | FFPE DNA | ≥50ng |
| Target capture sequencing of whole genome | cfDNA/ctDNA | ≥100pg |
| Whole genome sequencing | Microbial genome | 1ng-1μg |
| Whole genome sequencing(PCR-free) | High quality DNA | ≥50ng (no size selection) ≥200ng (size selection) |
| ChIP-Seq | ChIP DNA | ≥100pg |
| Targeted sequencing | Amplicon | ≥100pg |
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.
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