One-step Probe RT-qPCR Kit V2
High Quality One-Step Probe RT-QPCR Kit V2 V5009/V5010
Cat. No.: V5009,V5010
Components
| Component | V5009 (200 rxn, 30 μl/rxn) | V5010 (5000 rxn, 30 μl/rxn) |
| 2X RT-qPCR Mix * | 760 μl × 4 | 38.4 ml × 2 |
| Enzyme Mix b | 308 μl | 7.7 ml |
a. Contains dNTP/dUTP Mix, Mg2+.
b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.
Storage
This reagent should be kept at -20°C.
Description
The One-step Probe RT-qPCR Kit V2 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Thermostable reverse transcriptase can maintain stable activity at 55°C. The hot-start antibody-modified fast DNA polymerase and optimized buffer system ensure the extremely high sensitivity and specificity of the One Step Probe RT-qPCR Kit V2. The kit is available as a convenient Master Mix. 2 × RT-qPCR Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®.
Product Selection Guide
| Cat. No. | V5001/V5002 | V5005/V5006 | V5005L/V5006L | V5007/V5008 | V5007L/V5008L | V5009/V5010 | V5011 |
| Product Name | One-step Probe RT-qPCR Kit | DSPathTM4X One-Step Multiplex Master Mix | DSPathTM4X One-Step Multiplex Master Mix (Lyophilized) | 2X One Step Prime RT-qPCR Mix | 2X One Step Prime RT-qPCR Mix (Lyophilized) | One-step Probe RT-qPCR Kit V2 | One-step Probe RT-qPCR Kit V3 |
| Mixture Format | Enzyme Mix+2X Buffer Mix | 4X All-in Mix | 4X All-in Lyophilized Powder | 2X All-in Mix | 2X All-in Lyophilized Powder | Enzyme Mix+2X Buffer Mix | Enzyme Mix+5X Buffer Mix |
| Mode/Time | Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~88 min | Fast/~45min or Standard/~80min |
| RT Temperature | 50~55°C | 48~55°C | 48~55°C | 48~55°C | 48~55°C | 50~55°C | 50~55°C |
| PCR Enzyme | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq |
| Heat-labile UDG | + | + | + | + | + | + | + |
| Sensitivity | Very high | Very high | Very high | Very high | Very high | Ultra high | Ultra high |
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
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V5009 V5010 One Step RT QPCR Master Mix GDSBio RNA Reverse Transcription And Quantitative PCR Images
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