One-step Probe RT-qPCR Kit V3
High Quality 5× One-Step Probe RT-QPCR Kit V3 V5011
Cat. No.:V5011
Components
| Component | V5011 (200 rxn, 20 μl/rxn) |
| 5 × One-step U+ Mixa | 800 µl |
| One-step U+ Enzyme Mix V3b | 200 µl |
a. Contains dNTP/dUTP Mix, Mg2+.
b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.
Description
The One-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 5 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.
Storage
This reagent should be kept at -20°C.
Applications
• Probe gene expression analysis
• Probe Low-copy gene detection
• Probe microarray validation
• Probe gene knockdown validation
Features
• One-step RT-qPCR
• This kit is suitable for fluorescence quantification by probe method
• This kit is compatible with many real-time systems
• Hot-start technology brings high specificity and reproducible amplification
• dUTP/UDG system, effectively prevent PCR product contamination
Notes
1. One-Step U+ Enzyme Mix V3 contains high concentration of glycerol. Before use, please centrifuge briefly, collect to the bottom of the reaction tube, and gently suck with a pipetting gun.
2. For preparation of reaction solution, please use RNase-free pipette tips, EP tubes, etc., to avoid contamination as far as possible.
Product Selection Guide
| Cat. No. | V5001/V5002 | V5005/V5006 | V5005L/V5006L | V5007/V5008 | V5007L/V5008L | V5009/V5010 | V5011 |
| Product Name | One-step Probe RT-qPCR Kit | DSPathTM4X One-Step Multiplex Master Mix | DSPathTM4X One-Step Multiplex Master Mix (Lyophilized) | 2X One Step Prime RT-qPCR Mix | 2X One Step Prime RT-qPCR Mix (Lyophilized) | One-step Probe RT-qPCR Kit V2 | One-step Probe RT-qPCR Kit V3 |
| Mixture Format | Enzyme Mix+2X Buffer Mix | 4X All-in Mix | 4X All-in Lyophilized Powder | 2X All-in Mix | 2X All-in Lyophilized Powder | Enzyme Mix+2X Buffer Mix | Enzyme Mix+5X Buffer Mix |
| Mode/Time | Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~88 min | Fast/~45min or Standard/~80min |
| RT Temperature | 50~55°C | 48~55°C | 48~55°C | 48~55°C | 48~55°C | 50~55°C | 50~55°C |
| PCR Enzyme | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq |
| Heat-labile UDG | + | + | + | + | + | + | + |
| Sensitivity | Very high | Very high | Very high | Very high | Very high | Ultra high | Ultra high |
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
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5× Buffer RNA Reverse Transcription One Step Probe RT QPCR Master Mix V5011 Images
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