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Fast Restriction Endonuclease NdeI

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Guangzhou Dongsheng Biotech Co., Ltd

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Fast Restriction Endonuclease NdeI

Name: Fast Restriction Endonuclease NdeI
Brand: GDSBio
Price: 40 USD

Brand Name : GDSBio

Model Number : E1035

Certification : /

Place of Origin : China

MOQ : 100 rxns

Price : USD 40-44.5/100 rxns

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 100 Bag/Bags Per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Product Name : NdeI

Cat. No./Spec. : E1035-A/100 rxns, E1035-B/300 rxns

Appearance : colourless

Application : Molecular cloning

Classification : General Reagents

Grade : molecular biology

Logo Printing : With Logo Printing

Transport Package : Packing

Production Capacity : 100 Bag/Bags Per Day

Storage Conditions : Store at -20°C

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NdeⅠ

Cat. No./Spec.: E1035-A/100 rxns, E1035-B/300 rxns
Recognition site:
5' C A ↓ T A T G 3'
3' G T A T ↑ A C 5'

Product Description
The NdeⅠ restriction endonuclease recognizes the CA^TATG site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomer: FauNDⅠ.
GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.
The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.
The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.

Storage Condition & Shelf Life
Store at -20°C.

Features
- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.
- 100% buffer compatibility with downstream applications.
- Enzymatic digestion can be completed within 5–15 minutes.
- Direct loading onto a gel.
- No star activity.

Scope of Application
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP (Single Nucleotide Polymorphism) analysis

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