Product Introduction
SYBR® Green qPCR Mix with ROX is designed for high-performance, high-throughput real-time PCR. The kit contains Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that specifically designed for qPCR using SYBR® Green I chemistry dye.
2X SYBR® Green qPCR Mix with ROX is a convenient premix of the components (except primers and DNA template) that necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green I dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.
Application
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
Features
• Compatible with many Real-time systems whether requires ROX reference dye to calibration or not
• Hot-start technology brings high specificity and reproducible amplification
Components
| Component | P2091c | P2092c |
| 2X SYBR® Green qPCR Mix (with ROX+) | 1 ml | 1 ml × 5 |
| Nuclease-free Water | 1 ml | 1 ml × 5 |
Storage Condition
Store at -20°C and protected from light
Testing Data
SYBR Green qPCR Mix vs similar product from supplier T (global famous)
Table of Instrument Guide
| Instruments | Final Conc. of ROX |
| ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700 | 500 nM,high ROX |
| ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 | 50 nM,low ROX |
| Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler | No ROX |
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
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SYBR Green qPCR Mix with ROX for ABI Roche DNA real time PCR Images
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