Cat. No: P2201, 100 rxn/20μl reaction
P2202, 500 rxn/20μl reaction
HS Probe qPCR Mix P2201/P2202
For research use only
Components
| Component | P2201 | P2202 |
| 2× HS Probe qPCR Mix | 1 ml | 1 ml × 5 |
| Nuclease-free Water | 1 ml | 1 ml × 5 |
Storage
This reagent can be stored for 2 months at 4°C. For longer storage, it should be kept at -20°C.
Description
HS Probe qPCR Mix is designed for high-performance, high-throughput real-time PCR using sequence-specific fluorogenic probes. 2× HS Probe qPCR Mix contains the components (except primers, DNA template and probe) that necessary to perform PCR. The core component of the mix is OptimusTM Hotstart Taq DNA Polymerase, a hot-start polymerase with chemical modification, which brings high specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. Combined with well-optimized buffer can significantly improve amplification efficiency. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.
Applications
• Probe gene expression analysis
• Probe Low-copy gene detection
• Probe microarray validation
• Probe gene knockdown validation
Features
• This kit is suitable for fluorescence quantification by probe method
• This kit is compatible with many real-time systems
• Hot-start technology brings high specificity and reproducible amplification
Table of Instrument Guide
| Instrument | Conc. of ROX (100×) |
| ABI® PRISM® 7000, 7700, 7900HT, ABI® 7300 qPCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™ | 1-2% ROX |
| ABI® 7500 qPCR Systems, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™ Mx3005P™ and Mx4000™ | 0.2% ROX |
| BioRad iCycler MiniOpticon, Opticon 2, Chromo 4, iQ5; Roche LightCycler 480; MJ Research DNA Engine Opticon 2, Chromo 4; Corbett Rotogene 3000, 6000 | No ROX |
Human genome DNA (0.1ng/µl) was used as template for gene detection by Multiplex Probe qPCR Mix Plus U. FIG. 1 and FIG. 2 show the amplification curves of 0 hr (detected immediately after preassembly) and 48 hr (detected 48 hr after preassembly and being placed at RT), respectively. At the same time, a comparative test was conducted with similar products of manufacturer T. FIG. 3 and FIG. 4 show the amplification curves of manufacturer T. Table 1 shows the Ct values of Multiplex Probe qPCR Mix Plus U and manufacturer T at 0 hr and 48 hr. It can be seen that the Multiplex Probe qPCR Mix Plus U still has excellent amplification performance after being placed at room temperature for 48 hours.
Fig.1: P2701-0hr Fig.2: P2701-48hr
Fig.3: supplier T-0hr Fig.4: supplier T-48hr
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
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HS Probe qPCR Mix for DNA real time PCR Images
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