Power Green qPCR Mix
For research use only
Cat. No: P2101, 400 rxn/20μl reaction
P2102, 2000 rxn/20μl reaction
Components
| Component | P2101 | P2102 |
| 2X Power Green qPCR Mix | 1 ml | 1 ml × 5 |
| Nuclease-free Water | 1 ml | 1 ml × 5 |
Storage
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.
Description
Power Green qPCR Mix is a ready-to-use, 2X concentrated mix that contains all the reagents (except template and primers) needed for the real-time qPCR in the SYBR Green I detection format. This product is compatible with most manufacturers' real-time fluorescent quantitative PCR instruments such as Applied Biosystems, Roche, Bio-Rad, Eppendorf, Corbett and so on.
The Hotstart Taq DNA Polymerase in the mix is modified by antibody. When the temperature below 45°C, the activity of the modified polymerase will be strongly suppressed, only when the temperature reached 72°C, it can be activated. This technology helps to reduce non-specific amplification and primer dimer effectively.
Applications
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
Features
• Compatible with many Real-time systems which not require ROX reference dye
• Exceptional specificity with dual hot-start mechanism
• Tight reproducibility in Ct values over a broad dynamic range
• Universal instrument compatibility
Table of Instrument Guide
| Instruments | Final Conc. of ROX |
| ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700 | 500 nM,high ROX |
| ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 | 50 nM,low ROX |
| Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler | No ROX |
Testing Data:
Power Green qPCR Mix
Cat. No.: P2101/P2102
The Power Green qPCR Mix utilizes an antibody-modified hot-start Taq DNA polymerase to enhance the specificity of the reaction, ensuring the accuracy of gene quantification analysis. Specially optimized, this product allows for the preparation of the reaction system without the need for an ice box, and can be directly prepared at room temperature with components such as PCR Mix, primers, and templates. The prepared PCR reaction system can be kept at room temperature for 24 hours without changing the amplification efficiency, ensuring the stability of the workbench for large-scale testing.
qPCR Amplification Curve (left) and Melting Curve (right)
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
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Power Green qPCR Mix for DNA real time PCR 5ml Images
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