Direct Multiplex Probe QPCR Mix Plus U P2801 1ml
Cat. No: P2801, 66 rxn/30μl reaction P2802, 330 rxn/30μl reaction
P2803, 3333 rxn/30μl reaction P2804, 6666 rxn/30μl reaction
Direct Multiplex Probe qPCR Mix Plus U
For research use only
Components
| Component | P2801 | P2802 | P2803 | P2804 |
| 2× Direct Multiplex Probe qPCR Mix Plus U a | 1 ml | 1 ml × 5 | 50 ml | 100 ml |
| Nuclease-free Water | 1 ml | 1 ml × 5 | - | - |
a contains Hotstart Taq DNA Polymerase, dNTP/dUTP mix, UDG and reaction buffer.
Storage
This reagent can be stored for 2 months at 4°C. For longer storage, it should be kept at -20°C.
Description
Direct Multiplex Probe qPCR Mix Plus U is a 2× concentrated premix for unpurified sample real-time quantitative PCR with probe method. It requires only the addition of primer, probe and sample, such as blood, swab and tissue homogenate, and the qPCR can be performed simply and directly. This product contains antibody technology modified Hotstart Taq DNA polymerase. Combined with GDSBio's special real-time PCR Buffer, it not only efficiently lyses cells to release DNA, but also has a strong tolerance to many inhibitors, allowing multi-probe qPCR reaction. This reagent introduced dUTP/UDG anti-contamination system, which can remove PCR products containing dUTP before PCR reaction, effectively avoid the influence of cross contamination of amplification products on quantification. This product is convenient for preparation of IVD molecular diagnostic kit. The target gene quantification is accurate, reliable and reproducible. This product can be used with TaqMan and other fluorescent probes, and is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-rad, etc.
Applications
• Probe gene expression analysis with unpurified sample
Features
• This kit is suitable for fluorescence quantification by probe method
• This kit is compatible with many real-time systems
• Hot-start technology brings high specificity and reproducible amplification
• Direct PCR technology helps save time and cost
Table of Instrument Guide
| Instrument | Conc. of ROX |
| ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ Step One/ Step One Plus/ GeneAmp 5700 | 500 nM (High ROX) |
| ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 | 50 nM (Low ROX) |
| Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler | No ROX |
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
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